Download hornworm larvae7/15/2023 ![]() ![]() characterized StZFP1 and found it to be induced by Phytophthora infestans, salinity and abscisic acid. Only two ZFPs have been examined in potato. found that OEZat18 lines were more tolerant to drought stress while T-DNA mutant Zat18 lines were less tolerant. demonstrated that OEZat6 lines enhance tolerance to bacterial infection, freezing, drought and salinity stress, while artificial microRNA of Zat6 reduce tolerance. Over-expression of other ZFPs also results in an increase in tolerance to the stress that induces them. 8 This suggests that negative regulation of genes interacting with Zat7 play a part in the tolerance. For example, one of these ZFPs, OE-Zat7 enhances tolerance to salinity stress, while mutation of the EAR motif results in loss of that tolerance. 2, 7 When these ZFPs are over-expressed (OE), they can increase tolerance to that stress. ![]() Q-type C2H2 ZFPs have often been associated with environmental stress. 6 The zinc finger domain of these ZFPs bind specific genes while the EAR motif, as an active repressor, negatively regulates the gene that is bound to these ZFPs. ![]() ![]() 5 The corepressor in turn binds a histone deacetylase complex, which closes the chromatin surrounding the gene the TF has bound. Recent evidence from Arabidopsis has shown that the EAR domain in several ZFPs repress by binding a corepressor such as TOPLESS (TPL) or four TPL related corepressors. 3, 4 The repressor domain was named for the first protein in which it was identified, an ethylene-responsive element-binding factor (ERF)-associated amphiphilic repression or EAR domain. Another motif found in these ZFPs is the conserved DLN domain, which is an active repressor domain. The zinc bound domains interact with specific upstream regions of genes to control their expression. The Q-type C2H2 ZFPs (referred to here as ZFPs) have two zinc finger domains and these ZFPs are transcription factors (TF). 1, 2 The Q-type C2H2 is a thirty-amino acid zinc finger domain with an invariant QALGGH motif containing two Cys and two His residues that coordinate to bind a zinc ion. While there are 176 C2H2 zinc finger proteins (ZFPs) in Arabidopsis, 1 there are only 18 Q-type C2H2 ZFPs. StZFP2 is a member of the Q-type C2H2 zinc finger protein family. ![]()
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